Lineweaver-burk plot equation
NettetLineweaver-Burk Plot. Learn how the Lineweaver-Burk plot can be used to determine the type of inhibition on an enzyme. Explore the relationship between the equation and … NettetAbout Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright ...
Lineweaver-burk plot equation
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NettetCalculation of Kcat (the turnover number) If you have determined your Vmax (mM/min), the Kcat can easily be calculate by using following relation. Vmax = Kcat x [E]t. Kcat = Vmax/ [E]t. Where [E]t ... NettetEnzyme Kinetics : Lineweaver Burk Plot Explained Bio-Resource 20.9K subscribers Subscribe 2.7K views 2 years ago Bio-Resource: Lecture Videos This video explains about Enzyme Kinetics :...
Nettet10. jun. 2024 · Lineweaver-Burk plot gives a straight line for the rate data. y intercept = 1/ Vmax slope = Km/Vmax the x intercept = -1/ Km, too Problem MK5.2. Determine the values of Vmax and Km in each of the following cases. Assume the units of [S] are millimoles per liter and the units of V are moles per liter per second. Inhibition and … NettetHanes–Woolf plot. In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate …
NettetAbout Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright ... NettetPractice: A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the K m? Practice: Consider the equation for the line on the following …
NettetMoof's Medical Biochemistry Video Course: http://moof-university.thinkific.com/courses/medical-biochemistry-for-usmle-step-1-examFor Related Practice Problem...
http://www.biology.arizona.edu/BioMath/tutorials/Linear/LinearFunctionApplication/EnzymeKinetics.html days of rice and saltNettet17. feb. 2024 · The Lineweaver-Burk plot results in a straight line with the slope equal to \(K_M/k_2 \left[ \text{E} \right]_0\) and \(y\)-intercept equal to \(1/k_2 \left[ \text{E} … days of rivers pastNettet16. aug. 2024 · The double reciprocal plot (Lineweaver Burk plot) offers a great way to visualize the inhibition. In the presence of I, just Vm will decrease. Therefore, -1/Km, ... gccc flood reportNettetThe x-intercept in a Lineweaver-Burk plot corresponds to -1/Km, and the y-intercept corresponds to 1/Vmax. Therefore, we can use the values of Vmax and Km obtained … gcc central railwayNettetOne thing to note about the Lineweaver-Burk equation is that when plotted as a graph, it is a linear line with the equation y = mx + b (see Figure 1). As a line, interpreting the … days of riotsNettetConsider the equation for the line on the following Lineweaver-Burk plot: y = 6x + 3. A) What is the K m for the corresponding enzyme? a) -2 mM . b) 18 mM . c) 2 mM . d) 9 … gcc cep nursing programThe Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation, v = V a K m + a {\displaystyle v={\frac {Va}{K_{\mathrm {m} }+a}}} in which the rate v {\displaystyle v} is a function of the substrate concentration a {\displaystyle a} and two parameters V {\displaystyle V} , the limiting … Se mer In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934. The Lineweaver–Burk … Se mer $${\displaystyle [S]}$$: substrate concentration. The independent axis of a Lineweaver-Burk plot is the reciprocal of substrate concentration, Se mer When used for determining the type of enzyme inhibition, the Lineweaver–Burk plot can distinguish competitive, pure non-competitive Se mer • Michaelis–Menten kinetics • Eadie–Hofstee diagram • Hanes–Woolf plot Se mer The plot provides a very useful graphical method for analysis of the Michaelis–Menten equation, as it is difficult to determine precisely the Vmax of an enzyme-catalysed … Se mer While the Lineweaver-Burk is useful for determining important variables in enzyme kinetics, it is prone to error. The y-axis of the plot takes the reciprocal of the rate of reaction, meaning small errors in measurement are more noticeable. Additionally, the … Se mer • NIH guide, enzyme assay development and analysis Se mer gccc final schedule