WebPAmCherry is non-fluorescent until it is exposed to 350- to 400-nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest … WebOct 29, 2015 · Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved successful, pSC101 became the first plasmid cloning vector and molecular biology was never the same.
Addgene: pBAD-LSSmCherry1
WebArticle Snippet: pET-28a-hPKM1 plasmid (#44241) was purchased from Addgene Inc. (Watertown, MA, USA). mCherry-PKM2 plasmid (pCMV-PKM2-mCherry) was prepared by inserting the PKM2 gene (Clone ID hMU009169, Korea human gene bank) to pCMV-mCherry using Xho1 and EcoR1 sites. Techniques: Produced, Fluorescence. Journal: WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. fcf shop
LSSmCherry1 :: Fluorescent Protein Database
WebJan 3, 2024 · Plasmid vectors minimally consist of the transgene insert and an origin of replication, which allows for semi-independent replication of the plasmid in the host. Modern plasmids generally have many more features, notably a “multiple cloning site”—with nucleotide overhangs for insertion of an insert—and multiple restriction enzyme ... WebApr 1, 1986 · Plasmids are extrachromosomal pieces of double-stranded circular DNA which have the capability to replicate independently of the host chromosome, yet coexist with it [1]. To date, many species of bacteria isolated from diverse habitats are known to contain plasmid DNA [2,3]. Some plasmids are stable and can be maintained through successive ... WebWhen purifying DNA, it is critical to use an optimized method for your sample type. Our trusted DNA purification kits ensure high yields of high-quality DNA free of contaminants and inhibitors. Streamlined DNA extraction protocols simplify handling and are optimized for your specific sample types, formats and throughputs, as well as for manual ... fce heute