Pac2 cell
WebCell culture The zebrafish fibroblast cell line PAC2 was maintained as previously reported20. Cells were grown at 28 degrees Celsius in Leibovitz’s L-15 +glutamine Medium (Invitrogen, 21083027) containing 15% heat inactivated fetal bovine serum (FBS; Sigma-Aldrich, F4135-500ML) and 1% antibiotic-antimycotic (Corning, MT30004CI) until confluent. WebCellosaurus PAC2 (CVCL_5853) Group: Fish cell line. Omics: Transcriptome analysis. Derived from sampling site: Cell type=Fibroblast. Danio rerio (Zebrafish) (Brachydanio rerio) (NCBI Taxonomy: 7955 ) Random DNA integrations as an approach to insertional mutagenesis in the zebrafish (Brachydanio rerio). Genetic and transcriptome …
Pac2 cell
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WebATCC Primary Human Fibroblasts can be cultured in complete growth medium with or without serum. The use of Fibroblast Growth Kit–Serum-Free creates a completely defined medium for the serum-free culture of human fibroblasts. The rate of proliferation is equal to or greater than media supplemented using FBS (at concentrations ranging from 2% ... WebJun 21, 2024 · PAC2 cells were seeded in 96-well plates at 2.0 × 10 4 per well and the assay was performed accordingly to the manufacturer´s instructions. The optical …
WebZebrafish is an important model animal that has been used to study toxicology, developmental biology, and specific gene function and signaling pathways. Therefore, …
WebJun 1, 2012 · Foamy virus (FV) vector systems have recently demonstrated their power as efficient gene transfer tools for different target tissues. Unfortunately, FVs cannot be naturally pseudotyped by heterologous viral glycoproteins due to an unusual particle morphogenesis involving a FV Env-dependent particle release process. Therefore, … WebJun 7, 2010 · Zebrafish embryonic fibroblast (PAC2) cells were cultured as described before ( van Boxtel et al., 2008; Whitmore et al., 2000 ). To measure changes in gene expression, the cells were plated in 25-cm 2 tissue culture flasks (Greiner) and …
WebJun 1, 2009 · The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for …
WebSep 11, 2007 · PAC2 cells were electroporated with 5 μg each of linearized Cry1a-luciferase DNA and pcDNA3.1/myc-His A (Invitrogen, Carlsbad, CA). After neomycin selection, bioluminescence was monitored on a Packard TopCount scintillation counter (28°C), and cells from one strongly luminescent well were selected for these studies. furniture stores greenbeltWebPlasmid pAC2-dual-dCas9VP48-sgExpression from Dr. Rudolf Jaenisch's lab contains the insert dCas9VP48 and is published in Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. This plasmid is available through Addgene. furniture stores greenbrier chesapeake vaWebAug 22, 2024 · PAC3-PAC4 is required for formation of the core α4–α7 intermediate, whereas PAC1-PAC2 prevents its nonproductive dimerization. We also clarified that … furniture stores great western road glasgowWebIn constrast, PAC2 cells are capable of activating a promoterless neomycin gene at a frequency similar to that observed in cultured mouse cells. Analysis of the fusion transcript of one of the neomycin resistant PAC2 clones demonstrated that the neomycin resistance gene was activated by fusion with a gene normally expressed in PAC2 cells. giubbotto k-way uomo invernaleWebImpact of Light on Clock Genes in Cells. To explore how monochromatic light of selected wavelengths impacts gene expression in zebrafish cell lines, ClockDN and PAC2 cells were entrained, like the organs, on a 12:12 LD cycle at constant temperature and given a monochromatic light pulse for 3-hour at ZT21, when cells are most light responsive … giubbotti uomo the north faceWebFeb 1, 2013 · The immunoblotting of zebrafish CD from PAC2 cells resolved by 2D electrophoresis confirmed that zebrafish mature CD exists as a singular molecular specie of approximately 41 kDa with a computed isoelectric point of about 5.4 (Fig. 2 A).To see whether the lack of the β-hairpin loop in the primary sequence of zebrafish CD indeed … giubbotto tommy hilfiger uomoWebNov 15, 2024 · We next performed MTT (3- (4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) viability assays on the zebrafish embryo cell line PAC2. As shown in Figure 4, 72 h of treatment with up to 50 µM cyclopaldic and α-costic acid did not significantly reduce the viability of zebrafish cells. giubbotto north face nuptse