WebForbes D. Porter. Western blot relative quantification of FABP3 (A), CHM2A (B), GSTP1 (C), TRFE (D), TTR (E) and PSA1 (F). Validation was considered when differential … WebThe JESS capillary Western blotting is a technology for automated protein separation and immunodetection equivalent to those obtainable with traditional Western Blot, but with a number of advantages. The system exploits a technology with fully automated capillary microfluidics that provides for immobilization of the antigen directly in the capillaries.

Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary ...

Web• An optional System Control Primary Antibody (PN 042-196 or 042-191) can be mixed with your primary antibody in the assay to calculate inter-assay and inter-instrument variability (for chemiluminescence only). • You can use Bicine/CHAPS buffer (PN 040-764) or RIPA buffer (PN 040-483) to lyse your cells. Let’s get started! WebSep 22, 2024 · Protein Simple- Jess. Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels. Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. Just load your samples and reagents into the … black horse barnard castle

Has anyone used the ProteinSimple Wes capillary western blot …

WebForbes D. Porter. Western blot relative quantification of FABP3 (A), CHM2A (B), GSTP1 (C), TRFE (D), TTR (E) and PSA1 (F). Validation was considered when differential expression at one or more ... WebResolve proteins that are close in molecular weight with ease. The high-resolution 8.3-megapixel camera in the Amersham ImageQuant 800 system is capable of resolving even the most closely spaced bands, allowing for accurate quantitation in critical applications. In the example shown, it was possible to resolve bands that were just 0.5 mm apart. WebMar 3, 2024 · We employed an in vitro co-culture model system utilizing two different differentiated cell lines to mimic human intestinal tissue. ... Samples were denatured and equally loaded into a 12–230 kDa Jess/Wes Separation Module (ProteinSimple, Bio-Techne, Minneapolis, MN, USA, cat. no. SM W001). Primary antibodies were used for … black horse bar \u0026 grill winona